Use of different SEM techniques in the study of Tyrophagus putrescentiae (Acari: Acaridae) in Costa Rica

Pamela Murillo, Hugo Aguilar, Ethel Sanchez

Abstract


Scanning electron microscopy (SEM) has been used as a tool to facilitate the morphological description of mites and to complement the information obtained from the light microscope. Unfortunately, several of SEM techniques used for soft-bodied mites such as Tyrophagus, have in many cases not shown the desired results. This investigation sought to develop an efficient procedure for preparing specimens of T. putrescentiae for SEM so that its major morphological characters could be observed and photographed. This species is the most important contaminant of tissue culture laboratories in Costa Rica. Seven methods were used to process the samples. The evaluated treatments showed differences in their ability to preserve the morphological structures of the specimens. The advantages and disadvantages of each treatment are discussed.  Mites processed using ethanol were the most viable option with method 3 being the procedure that showed the best results; this technique also reduced the time and costs of processing the specimens.

KEY WORDS

Acari, Acaridae, Tyrophagus putrescentiae, Scanning Electron Microscopy (SEM), SEM techniques


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References


Achor, D.S. & Childers, C. (1995). Fixation techniques for observing thrips morphology and injury with electron microscopy. In: B.L. Parker, M. Skinner & T. Lewis, (Eds.), Thrips biology and management (pp 595-600). New York, NY: Plenum Press.

Achor, D., Ochoa, R., Erbe, E., Aguilar, H., Wergin, W. & Childers, C. (2001). Relative advantages of low temperature versus ambient temperature scanning electron microscopy in the study of mite morphology. International Journal of Acarology, 27(1), 3-12.

Aguilar, H., Murillo, P. & Gómez, L.E. (2006). Role of mites in tissue culture laboratories: the case of Siteroptes reniformis in Costa Rica. Abstract Book, 12th International Congress of Acarology 21-26 August, 2006, Amsterdam, The Netherlands.

ARS-USDA. (2000). Mites get frozen, photographed, and identified. Agriculture Research Magazine, 48(10), 4-7.

ARS-USDA. (2007). A Tiny Menace Island-Hops the Caribbean. Agriculture Research Magazine, 55(5), 4-7.

Beard, J.J., Ochoa, R., Bauchan, G., Welbourn, W.C., Pooley, C. & Dowling, A.P.G. (2012). External mouthpart morphology in the Tenuipalpidae (Tetranychoidea): Raoiella a case study. Experimental and Applied Acarology, 57(3-4), 227-255.

Blake, J. (1988). Mites and thrips as bacterial and fungal vectors between plant tissue cultures. Acta Horticulture, 225, 163- 166.

Boxus, P.H. & Terzi, J.M. (1987). Big losses due to bacterial contamination can be avoided in mass propagation scheme. Acta Horticulture, 212, 91-93.

Dowling, A.P.G., Bauchan, G.R., Ochoa, R. & Beard, J. (2010). Scanning Electron Microscopy vouchers and genomic data from an individual specimen: maximizing the utility of delicate and rare specimens. Acarology, 50(4), 479–485.

Duek, L., Kaufman, G., Palevsky, E. & Berdicevsky, I. (2001). Mites in fungal cultures. Mycoses, 44, 390- 394.

Erbe, E.F., Wergin, W.P., Ochoa, R., Dickens, J.C. & Moser, B. (2001). Low temperature scanning electron microscopy of soft bodied organisms. Proceedings of the Microscopy Society of America, Microscopy and Microanalysis, 7(2), 1204-1205.

Fan, Q.H. & Zhang, Z.Q. (2007). Tyrophagus (Acari: Astigmata: Acaridae). Fauna of New Zealand (pp 14- 291). New Zealand, NZ: Manaaki Whenua Press, Lincoln.

Hughes, A.M. (1976). The mites of Stored Food and Houses. Technical Bulletin No.9, Ministry of Agriculture, Fisheries and Food (p 399). London: Her Majesty´s Stationary Office.

Keirans, J.E., Clifford, C.M. & Corwin, D. (1976). Ixodes sigelos, n. sp. (Acarina: Ixodidae), a parasite of rodents in Chile, with a method for preparing ticks for examination by scanning electron microscopy. Acarology, 18(2), 217-25.

Klimov, P.B. & OConnor, B.M. (2009). Conservation of the name Tyrophagus putrescentiae, a medically and economically important mite species (Acari:Acaridae). International Journal of Acarology, 35(2), 95-114.

Kucerova, Z. & Stejskal, V. (2009). Morphological diagnosis of the eggs of stored-products mites. Experimental and Applied Acarology, 49(3), 173-183.

Leifert, C., Morris, C. & Waites, W.M. (1994). Ecology of microbial saprophytes and pathogens in tissue cultured and field grown plants. CRC Critical Reviews in Plant Sciences, 13, 139-89.

Leifert, C. & Cassells, A.C. (2001). Microbial hazards in plant tissue and cell cultures. In Vitro Cellular and Development Biology, 37, 133-138.

Mariana, A., Santana Raj, A.S., Ho, T.M., Tan, S.N. & Zuhaizam, H. (2008). Scanning Electron Micrographs of two species of Sturnophagoides (Acari: Astigmata: Pyroglyphidae) mites in Malaysia. Tropical Biomedicine, 25(3), 217–224.

Ochoa, R., Erbe, E.F., Wergin, W.P., Frye, C. & Lydon, J. (2001). The presence of Aceria anthocoptes (Nalepa) (Acari: Eriophyidae) on Cirsium species in the United States. International Journal of Acarology, 27, 179-187.

Ojeda-Sahagún, J.L. (1997). Métodos de microscopia electrónica de barrido en biología, (p 267). Santander: Universidad de Cantabria, D.L.

Postek, M., Howard, K., Johnson, A. & McMichael, K. (1980). Scanning electron microscopy, ( p 305). USA: Ladd Research Industries, Inc.

Serageldin, I. (1999). Biotechnology and food security in the 21st century. Science, 285, 387-389.

Valdez, M., López, R. & Jiménez, L. (2004). Estado actual de la Biotecnología en Costa Rica. Revista de Biologia Tropical, 52(3), 733-743.

Wergin, W., Ochoa, R., Erbe, E., Craemer, C. & Raina, A. (2000). Use of low- temperature field emission scanning electron microscopy to examine mites. Scannig, 22(3), 145-155.

Wergin, W., Erbe, E. & Ochoa, R. (2001). Versatile and inexpensive specimen holder for high angle azimuth rotation in a low temperature scanning electron microscope. Proceedings of the Microscopy Society of America, Microscopy and Microanalysis, 7(2), 718-719.

Zhang, Z.Q. (2003). Mites of Greenhouses: Identification, Biology and Control, (p 244). Wallingford, UK: CABI Publishing.




DOI: http://dx.doi.org/10.22458/urj.v5i2.273

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